The dope on l ‐ DOPA formation for betalain pigments

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Genetic engineering of yellow betalain pigments beyond the species barrier

Betalains are one of the major plant pigment groups found in some higher plants and higher fungi. They are not produced naturally in any plant species outside of the order Caryophyllales, nor are they produced by anthocyanin-accumulating Caryophyllales. Here, we attempted to reconstruct the betalain biosynthetic pathway as a self-contained system in an anthocyanin-producing plant species. The c...

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Oleh Hornykiewicz was born in 1926 in Sykhiw, Poland (now Ukraine). In 1951, he received his M.D. degree from the University of Vienna and joined the faculty of his alma mater the same year and has worked there ever since. He also served for twenty years as chairman of the Institute of Biochemical Pharmacology. In 1967, he began a long association with the University of Toronto in Canada and, i...

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Lineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllales

Betalain pigments are unique to the Caryophyllales and structurally and biosynthetically distinct from anthocyanins. Two key enzymes within the betalain synthesis pathway have been identified: 4,5-dioxygenase (DODA) that catalyzes the formation of betalamic acid and CYP76AD1, a cytochrome P450 gene that catalyzes the formation of cyclo-DOPA. We performed phylogenetic analyses to reveal the evol...

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Cytosolic and Nuclear Co-localization of Betalain Biosynthetic Enzymes in Tobacco Suggests that Betalains Are Synthesized in the Cytoplasm and/or Nucleus of Betalainic Plant Cells

Betalains replace anthocyanins as color pigments in most families of Caryophyllales. Unlike anthocyanins, betalains are derived from tyrosine via three enzymatic steps: hydroxylation of L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA; step 1), and conversion of L-DOPA to betalamic acid (step 2), and to cyclo-DOPA (cDOPA; step 3). The principal enzymes responsible for these reactions have bee...

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ژورنال

عنوان ژورنال: New Phytologist

سال: 2016

ISSN: 0028-646X,1469-8137

DOI: 10.1111/nph.13901